･Viral vectors or deconstructed viral vector strategies result in enhanced recombinant protein yield, high throughput, and scalability.
･We have developed the “Tsukuba system” in which the expression level of the green fluorescent protein (GFP) is much faster than the existing transient expression systems.
･We have also found not only to increase the yield of proteins, optimization of codon usage is recommended for transient expression, but also to prevent necrosis using a foliar spray application with high concentrations of ascorbate due to overexpression of recombinant proteins in plant cells.
