･To make reproducible spot assay experiments widely available, we have automated the plate-based yeast spot assay of budding yeast using Opentrons OT-2, an affordable liquid-handling robot, and a flatbed scanner.
･Our proposed method can perform the entire process including the dilution series preparation, spotting, and continuous observation of yeast growth, contributing to improved reproducibility of experimental manipulations, reduction of human errors, and traceability of results.
･This research is expected to contribute to the promotion of decentralized laboratory automation by lowering the barrier to entry for laboratory automation in a broad range of wet labs.
