･We set out to determine whether histidine methylation occurs in histone using a unique method of analyzing methyl amino acids derived from hydrolysate of histone proteins combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
･MALDI-TOF MS analysis revealed that histones H2A and H3 were methylated at His-82 and His-39, respectively.
･Our findings provided evidence that histones indeed carry a τMH modification and suggest that this methylation has additional implications for the histone code hypothesis.
